Archives

VOLUME 3 NO. 1 , 2019

ONLINE FIRST

Transplantation of Bone Marrow–Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Fibrin Glue for Periodontal Regeneration
Mahdieh Rezaei / Shahram Jamshidi / Anna Saffarpour / Mahdi Ashouri / Reza Rahbarghazi / Amir Reza R

Transplantation of Bone Marrow–Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Fibrin Glue for Periodontal Regeneration

Appropriate regeneration ofperiodontal tissues is the primary purpose of periodontal disease treatment.The present study assessed the impact of three key regenerativeelements—platelet-rich plasma (PRP), canine bone marrow mesenchymal stem cells(cBM-MSCs), and fibrin glue—on periodontal regeneration. In each of the study’sfive dogs, Class II furcation defects were established on the buccal surface offive teeth. One tooth (five total) from each dog was placed into one of fivegroups: (1) PRP + fibrin glue, (2) PRP + fibrin glue + cBM-MSCs, (3) fibringlue, (4) cBM-MSCs + fibrin glue, and (5) control (no treatment). Histologicand histometric evaluations were performed to assess the formation of new bone,cementum, and the periodontal ligament. Different types of new bone andcementum, the maximum thickness of new cementum and the periodontal ligament(PDL), the vitality of bone, and the presence of inflammation or foreign-bodyreactions were also elucidated. The histologic and histometric evaluationsrevealed substantial differences in all groups between the observed maximumthickness of newly formed cementum and PDL. The percentage of bone and cementformation drastically increased with the combined presence of stem cells,fibrin glue, and PRP. The results showed that the inherent regenerativecapacity of periodontal tissues can be sufficient if their latent self-repairmechanisms are stimulated.